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Recombinant enzyme human chymotrypsin proteolytic enzyme CAS 9004-07-3 EC 3.4.21.1

RCT10

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Description

Recombinant?Chymotrypsin

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DESCRIPTION

Chymotrypsin?is?a?recombinant?serine?endopeptidase?expressed?in?E.coli,?purified?with?HPLC,?the?gene

?sequence?is?same?as?that?human?chymotrypsin.?The?recombinant?chymotrypsin?hydrolyzes?at?the?carboxyl?

side?of?aromatic?amino?acids:?Tyr,?Phe?and?Trp.?Cleavage?may?also?be?observed,?but?at?a?lower?rate,?at?

Leu?and?Met.?Chymotrypsin?activity?is?optimal?in?the?pH?range?of?7.0–9.0.?This?sequencing?grade?enzyme

?can?be?used?alone?or?in?combination?with?other?proteases?to?produce?protein?digests?for?peptide?mapping?

applications?or?protein?identification?by?peptide?mass?fingerprinting?or?MS/MS?spectral?matching.?It?is?

suitable?for?digestion?reactions?in-solution?or?in-gel.

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ADVANTAGES

Animal?origin?free:The?use?of?recombinant?chymotrypsin?eliminates?the?content?of?other?proteins,?the

?risk of?virus?presence,?and?other?potential?adventitious?agents?found?in?animal-derived?chymotrypsin.?

Stable:A?sterile?recombinant?chymotrypsin?lyophilized?powder?eliminates?the?contamination?risks?and?

decreases?the?chance?of?activity?loss?in?the?process?of?transport?and?storage.

High?purity:1)Recombinant?Chymotrypsin?provides?increased?specificity?and?eliminates?contaminating?

???????????????activities?found?in?enzymes?purified?from?pancreas.

?????????????2)?No?anion?trypsin?or?cation?trypsin.

?????????????3)?One?single?main?band?at?25kD?by?SDS-PAGE.

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SPECIFICATION

Serine?endopeptidase?that?specifically?hydrolyzes?peptide?bonds?at?the?C-termini?of?Tyr,?Phe,?and?Trp.?Leu,

?Met,?Ala,?Asp,?and?Glu?are?cleaved?at?a?lower?rate.?Acts?also?upon?amides?and?esters?of?susceptible?amino

?acids.

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APPLICATION

Hydrolysis?of?proteins?by?chymotrypsin?alone?or?in?combination?with?other?proteases.?Suitable?for?peptide?

mapping,?fingerprinting,?and?sequence?analysis.

For?peptide?digestion,?use?a?ratio?(w/w)?of?approximately?1:60?for?chymotrypsin:peptide.?Perform?peptide?

digests?in?100?mM?Tris?HCl?containing?10?mM?CaCl2,?pH?7.8,?at?30?°C.?Self?digestion?may?occur?if?

temperatures?above?37?°C?are?used.?A?known?peptide?such?as?melittin?should?be?used?as?a?control?for?all?

experiments.

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MAIN?FEATURES?

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Source | E.?Coli |
Purified?by | HPLC? |
Physical?form | White?lyophilized?powder |
Specific?activity | ≥1000?unit/mg?pro |
Purity | >?99% |
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PRODUCT?INFORMATION

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Product | Cat.?No. | Specific?activity | Packaging | Manufacturer |
Recombinant?Chymotrypsin | RCT10 | ≥1000?unit/mg?pro | 1mg,100mg,1g?or?bulk | YaxinBio |
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UNIT?DEFINITION:?One?unit?will?hydrolyze?1.0?μmole?of?BTEE?per?min?at?pH?7.8?at?25?°C.

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Recombinant?Trypsin

V8

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