Adhesives & Sealants
Description
recombinant human trypsin lyophilized powder,human trypsin supplier
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Recombinant?Human?Trypsin?
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DESCRIPTION
Human Trypsin?
Trypsin?is?a?member?of?the?serine?protease?family.?Trypsin?cleaves?peptides?on?the?C-terminal?end?of?lysine
?and?arginine?amino?acid?residues.?Recombinant?Human?Trypsin?is?a?genetically?engineered?protein,?
expressed?in?E.Coli?and?purified?by?high?pressure?liquid?chromatography.?The?amino?acid?sequence?is?the
?same?as?Human?Trypsin?2?sequence.?The?pH?optimum?of?trypsin?is?7?-?9.?The?enzyme?is?inhibited?by?serine
?protease?inhibitors,?e.g.?PMSF,?and?by?metal?chelating?agents,?e.g.,?EDTA.?There?are?no?contaminating
?enzyme?activities?such?as?from?carboxypeptidase?A?and?chymotrypsin.?No?protease?inhibitors?such?as?
PMSF?are?contained?in?the?preparation.
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ADVANTAGES
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Animal?origin?free:The?use?of?recombinant?Human?Trypsin?eliminates?the?risk?of?virus?presence,?and?of?any
other?potential?adventitious?agents?found?in?animal-derived?trypsin.?YaxinBio?Recombinant?Human?Trypsin?belongs?to?the?AOF?level?3.
Recombinant?human?trypsin:The?amino?acid?sequence?is?the?same?as?the?Human?Trypsin?2?sequence.
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Stable:A?sterile?recombinant?trypsin?lyophilized?powder?eliminates?the?contamination?risks?and?decreases
?the?chance?of?activity?loss?in?the?process?of?transport?and?storage.
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High?purity:
# # Recombinant?human?trypsin?provides?increased?specificity?and?eliminates
contaminating?activities?found?in?lower?purity?enzymes.
2)?No?other?contaminating?proteases?such?as?chymotrypsin?or?carboxypeptidase?A.
3)?One?single?main?band?at?24kD?by?SDS-PAGE.
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Figure?1.?SDS?Page?of?YaxinBio?Recombinant?Human?Trypsin?(rhT)
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Source? | E.?Coli |
Purified?by | HPLC |
Physical?form | White?or?White-like?lyophilized?powder |
Specific?activity | ≥2500?USP?u/mg?pro. |
Purity | >?99% |
Contaminant?activity | No?chymotrypsin,?carboxypeptidase?A,?and?other?protease?contaminant.? |
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MAIN?FEATURES
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PRODUCT?INFORMATION
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Product | Cat.?No. | Specific?activity | Packaging | Manufacturer |
Recombinant?Human?Trypsin?2 | RHT03 | ≥2500?USP?u/mg?pro | 10mg,100mg?or?bulk | YaxinBio |
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UNIT?DEFINITION:One?USP?unit?of?trypsin?activity?will?produce?a?Delta?A253?of?0.003?per?minute?in?a
?reaction?volume?of?3.0ml?at?pH7.6?and?25℃,?with?BAEE?as?a?substrate?(1cm?light?path).
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Shanghai Yaxin Biotechnology Co.,Ltd.
Recombinant Trypsin,Sequencing Grade Trypsin,Recombinant Carboxypeptidase B
Address: No.466 YinDu Road,XuHui, Shanghai, China,
Shanghai, Shanghai
China, 200000
Tel: 86-021-54336592
Fax: 86-021-54336593