Biochemical
Description
Apramycin is an aminoglycoside antibiotic used in veterinary medication. The strong anti-bactericidal
activity focuses on gram-negative bacteria (Escherichia coli, Pasteurella), Staphylococcus and
Mycoplasma with binding 30s ribosomal subunit to inhibit protein synthesis. Apramycin ELISA kits
utilizing the latest biotechnology development has easy, rapid, and sensitive advantages.
This kit uses indirect-competition method mainly based on the immunological reaction between specific
antigen and antibody. Apramycin specific antigen coated on microtiter plates competes binding sites of
enzyme-labeled Apramycin specific antibody with added calibrator (or sample). With TMB chromogenic
substrate, the absorbance value was negatively correlated with residues of Apramycin. Under the
calibration curve, Apramycin content of the sample can be quantified multiplied by the corresponding
compare dilution.
Detectable samples
Apramycin residue can be quantitative of tissue, liver and honey.
Specificity
Apramycin-----------------------------------------------100%
spectinomycin, streptomycin, kanamycin, tilmicosin <0.1%
Detection limit:
0.1ppb is the lowest detection limit of Apramycin kit. Linear regression is between 0.1-8.1ppb.
Sensitivity:
1. Tissue, Liver ----------------------------------------------5ppb
2. Honey ------------------------------------------------------2ppb
Recovery rate:
1. Tissue -------------------------------------------------60-120%
2. Liver----------------------------------------------------60-120%
3. Honey -------------------------------------------------60-120%
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SHENZHEN WANYUANSHENG TECHNOLOCY CO.,LTD
Food safety ELISA diagnostic kit,Tetracycline ELISA Diagnostic Kit,AOZ ELISA Diagnostic Kit
Address: BLOCK 202#13 LANE B AREA HUAYUANPING AILIAN COMMUNITY LONGCHENG STREET LONGGANG DISTRICT SHENZHEN,
shenzhen, Guangdong
China, 518172
Tel: +8675589565605
Fax: +8675589565605
