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sequencing grade recombinant trypsin / trypsin for in-gel protein digestion

SRT0202

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Description

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Sequencing Grade TrypsinTrypsin / SRT / sequencing grade enzyme

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CAS:?9002-07-7


EC:?3.4.21.1

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SOURCE:Expressed?in?E.?Coli,?purified?by?HPLC.

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DESCRIPTION

Sequencing Grade Trypsin

Trypsin?is?a?member?of?the?serine?protease?family.?Trypsin?cleaves?peptides?on?the?C-terminal?end?of?lysine

?and?arginine?amino?acid?residues.?The?pH?optimum?of?trypsin?is?pH?7?-?10.?The?enzyme?is?inhibited?by

?serine?protease?inhibitors,?e.g.?PMSF,?and?by?metal?chelating?agents,?e.g.,?EDTA.

Recombinant?Porcine?Trypsin?is?a?genetically?engineered?protein?expressed?in?E.coli?and?purified?by?high

?pressure?liquid?chromatography.?There?is?no?trace?of?contaminating?enzyme?activities?such?as?from

?carboxypeptidase?A?and?chymotrypsin.?No?protease?inhibitors?such?as?PMSF?are?contained?in?the

?preparation.

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MAIN?FEATURES?

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Source | E.?Coli |
Purified?by | HPLC |
Physical?form | Lyophilized?powder |
Specific?activity | >4500?USP?units/mg?pro |
Purity | One?single?main?band?at?24kD?by?SDS-PAGE |
Contaminant?activity | No?chymotrypsin,?carboxypeptidase?A,?or?other?protease?contaminant.? |
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PRODUCT?INFORMATION

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Product | Cat.?No. | Specific?activity | Packaging | Manufacturer |
Recombinant?Trypsin | SRT0202 | ≥4500?USP?/mg?pro | 100ug??1mg? | YaxinBio |
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RELATED?PRODUCT

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Recombinant?Trypsin?(sequencing?grade);

Recombinant?Carboxypeptidase?B?(sequencing?grade);

Recombinant?Chymotrypsin?(sequencing?grade);

V8?(sequencing?grade).

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