Biochemical
Description
The method is based on a competitive colorimetric ELISA assay. The plate wells are coated with capture antibodies directed against anti- sulfonamide antibodies. Sulfonamide standards or sample solution, sulfonamide–HRP conjugate and anti-sulfonamide antibodies are added. Free sulfonamide and sulfonamide-HRP Conjugate compete for the sulfonamide antibody binding sites. At the same time, the anti- sulfonamide antibodies are also bound by the immobilized capture antibodies. Any unbound sulfonamide-HRP Conjugate is then removed in a washing step. The TMB Substrate is added to the wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
elisa kit, Sulfonamide,Read More
REAGEN LLC
elisa kit
Address: 102 Commerce Dr. Unit 8, Moorestown, NJ 08057,
Moorestown, New Jersey
United States, 08057
Tel: 1-856-6711789
Fax: 1-856-8585434
