Bio-technology Products
Description
Ractopamine ELISA Test Kit
Catalog No. LSY-10013
1. Principle
The test kit is based on the competitive enzyme immunoassay for the detection of Ractopamine in the sample. The conjugated antigens is pre-coated on the micro-well stripes. The Ractopamine in the sample and the coupling antigens pre-coated on the micro-well stripes compete for anti-Ractopamine antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Ractopamine concentration in the sample. The value is compared to the standard curve and the Ractopamine concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.04 ppb
Incubator temperature: 25℃
Incubator time: 30min～15min
Detection limit
Urine 0.04ppb
Tissue……………………………………………………………………… 0.16 ppb
Feed 0.4 ppb
Cross-reactions
Ractopamine 100%
Dobutamine <1%
Salbutamol <0.1%
Clenbuterol <0.1%
Recovery rate
Urine 95±18%
Tissue 95±10%
Feed 85±15%
3. Components
1）Micro-well strips: 12 strips with 8 removable wells each
2）6× standard solution (1 mL each): 0 ppb, 0.04 ppb, 0.12ppb, 0.36 ppb, 1.08 ppb, 3.24 ppb
3）Enzyme conjugate (7 mL) red cap
4）Antibody working solution (10 mL) blue cap
5）Substrate A solution (7 mL) white cap
6）Substrate B solution (7 mL) black cap
7）Stop solution (7 mL) yellow cap
8)20× concentrated washing buffer (40 mL) white cap
9)50× concentrated redissolving solution (50 mL) transparent cap
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Shenzhen Lvshiyuan Biotechnology Co.,Ltd
food safety elisa test kit
Address: D building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Longgang, Shenzhen,518120, China,
Shenzhen, Guangdong
China, 518120
Tel: 86-755-28438788
Fax: 86-755-28938800
